KMID : 0354720070310030230
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Journal of Korean Diabetes Association 2007 Volume.31 No. 3 p.230 ~ p.235
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Effects of Lovastatin on Free Fatty Acid Oxidation in Cultured L6 Rat Skeletal Muscle Cells
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Kim Dong-Lim
Song Kee-Ho Kim Hae-Rim Kim Suk-Kyeong
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Abstract
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Background: Recent clinical studies suggest that statins improve insulin resistance and glucose metabolism
in patients with metabolic syndrome and type 2 diabetes. To evaluate the possible mechanism of this action, we measured free fatty acid oxidation in cultured L6 rat skeletal muscle cell line.
Methods: Cultured L6 myotubes were treated with or without lovastatin (1, 5, 20 ¥ìM) for 24 hours or 48 hours and palmitate oxidation was measured. We also measured protein concentration of the cells.
Results: Lovastain increased palmitate oxidation in dose and time dependent manner in L6 myotubes (24 hr;
1 ¥ìM 119.2 ¡¾ 11.9% of control, 5 ¥ìM 140.9 ¡¾ 8.1%, 20 ¥ìM 150 ¡¾ 5%, P = 0.05 vs control, respectively, 48 hr 1 ¥ìM 120.9 ¡¾ 14.5%, 5 ¥ìM 176.6 ¡¾ 28.2%, 20 ¥ìM 196.0 ¡¾ 19.9%, P < 0.01 vs control, respectively). However, lovastatin decreased total cellular protein (24 hr: 1 ¥ìM 89.2 ¡¾ 6.1% of control, 5 ¥ìM 79.3 ¡¾ 7.6%, 20 ¥ìM 65.4 ¡¾ 4.2%, P = 0.05 vs control, respectively, 48 hr: 1 ¥ìM 81.7 ¡¾ 5.1%, 5 ¥ìM 58.6 ¡¾ 11.9%, 20 ¥ìM 48.1 ¡¾ 6.9%, P < 0.01 vs control, respectively).
Conclusion: Lovastatin increased skeletal muscle free fatty acid oxidation in L6 rat skeletal muscle cells. This
would be one of the mechanisms which lovastatin improves insulin resistance.
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KEYWORD
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Fatty acid oxidation, Skeletal muscle, Statins
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